Alignment using bwa-mem2
Hello
I need help in aligning the sequence with reference using bwa-mem2. I used the following code:
bwa-mem2 mem -t 8 gch38.fa DE98NGSUKBD117612_1_1.fq DE98NGSUKBD117612_1_2.fq > d3_align.sam
I got the following error:
ERROR! Unable to open the file: gch38.fa.bwt.2bit.64
There is no gch38.fa.bwt.2bit.64 file. I have the following the reference indexes:
gch38.fa gch38.fa.0123 gch38.fa.amb gch38.fa.ann gch38.fa.pac
What is the gch38.fa.bwt.2bit.64 file?
Thank you
Priya
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