STAR split reads into two strands

STAR split reads into two strands

0

Hi all,

I’m trying to find a way to split reads mapped with STAR into two separate bam-files, one for each strand. STAR is able to do that when creating bedgraph or bigwig outputs (–outWigType and –outWigStrand), however I can not find any option to split raw reads into two separate files correponding to the strand they were transcribed from. I do not want to get counts per gene, I know how to do that, instead I need to get actual reads for each strand. Any ideas?

Thanks a lot in advance


rnachip


stranded


split


STAR

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