cellranger count help

cellranger count help

0

enter image description here

these were original data from sequencing company

and then i compressed these files into two files R1 and R2
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/data01/chenyu/sc/cellranger-6.1.1/cellranger count
–id=cellranger_szdxb015
–fastqs=/data01/chenyu/sc/sortData/191527A_SZdxb01_5
–sample=SZdxb015
–transcriptome=/data01/chenyu/sc/refdata-gex-GRCh38-2020-A
–localcores=20
–nosecondary

error occured:

FASTQ header mismatch detected at line 4 of input files “/data01/chenyu/sc/sortData/191527A_SZdxb01_5/SZdxb015_S1_L001_R1_001.fastq.gz” and “/data01/chenyu/sc/sortData/191527A_SZdxb01_5/SZdxb015_S1_L001_R2_001.fastq.gz”: file: “/data01/chenyu/sc/sortData/191527A_SZdxb01_5/SZdxb015_S1_L001_R1_001.fastq.gz”, line: 4

how to fix this problem
thanks in advance


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