What are the steps of an full lengh RNA seq annotation method?
I have read a few papers, some of them used GenMark, or PacBios proprietary Iso-Seq pipeline but as I’m just a Bachelor’s student I am having trouble understanding how exactly it works.
Can some one roughly explain the steps of a state of the art pipeline that I could apply to an invertebrate?
The data of my cnidarian species should be annotated with help of PacBio RNA seq data.
Your insights are much appreciated!
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