I downloaded fastq files from a repository and tried to run fastqc, how can the average sequence length be only 8 bp?
I downloaded sequencing files from 2 patients from here:
www.ebi.ac.uk/ena/browser/view/PRJNA588461?show=reads
there is one fastq file for the forward (1) and reverse (2) reads.
I wanted to look at the quality of the data using fastqc, which I ran simply with fastqc *fastq.gz
The result was that the average forward seuqnence length was 8 pb while the average reverse sequence length was 76. How is this possible?
I am new the analyzing scRNAseq data, perhaps theres a huge step I am missing?
Thanks!
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