RSEM problem

RSEM problem

0

Good day all!
I’m running RSEM as a batch job on a cluster:

rsem-prepare-reference
–gtf /home/tbeckett/lustre/honours_project/RSEM/ref/Mus_musculus.GRCm39.103.chr.gtf
–bowtie2 –bowtie2-path software/bowtie2/2.3.4
/home/tbeckett/lustre/honours_project/RSEM/ref/Mus_musculus.GRCm39.dna.toplevel.fa /home/tbeckett/lustre/honours_project/RSEM/ref/mouse_ref

I recieved an error:
Transcript ENSMUST00000132294 is out of chromosome 7’s boundary!

Any idea what this error might be?

Also, the second line when specifying bowtie, software/bowtie2/2.3.4, what is the point of specifying ‘software’? im copying it from a tutorial.

Help will be appreciated!


RSEM


Mapping

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