Tag: GEO

Visiongain Reports Ltd Visiongain has published a new report entitled: Translational Regenerative Medicine Market Report 2024-2034: Forecasts by Product (Stem Cell Therapy (Autologous, Allogenic), Tissue Engineering (Scaffold, Hydrogels), Gene Therapy, Others)), by Application (Oncology, Dermatology, Musculoskeletal, Neurology, Cardiovascular, Wound Healing, Ophthalmology, Others) AND Regional and Leading National Market Analysis PLUS…

Visiongain has published a new report entitled: Translational Regenerative Medicine Market Report 2024-2034: Forecasts by Product (Stem Cell Therapy (Autologous, Allogenic), Tissue Engineering (Scaffold, Hydrogels), Gene Therapy, Others)), by Application (Oncology, Dermatology, Musculoskeletal, Neurology, Cardiovascular, Wound Healing, Ophthalmology, Others) AND Regional and Leading National Market Analysis PLUS Analysis of Leading…

Dear all, I’m trying to carry out umbrella sampling for a dimeric system. My collective variable is center of mass to center of mass distance. The COLVAR and CONSTRAINT section is as follows. I’m getting different error with different modifications. Can anyone suggest me how to correct it ? Thanks…

Introduction Combined allergic rhinitis and asthma syndrome (CARAS), a new terminology introduced by the World Allergy Organization (WAO) in 2004, is an allergic reaction that occurs in the respiratory tract, including upper respiratory tract allergy (allergic rhinitis, AR) and lower respiratory tract allergy (asthma, AS).1,2 The incidence of AS in…

Visiongain has published a new report entitled Viral Vectors & Plasmid DNA Manufacturing Market Report 2024-2034: Forecasts by Vector Type (Adenovirus, Retrovirus, Plasmid DNA, AAV, Lentivirus, Others), by Application (Antisense and RNAi, Gene Therapy, Cell Therapy, Vaccinology), by End-use (Pharma and Biopharma Companies, Research Institutes), by Disease (Oncology, Genetic Disorders,…

Loss of ASH2L prevents cell proliferation We have studied the molecular and cellular consequences of Ash2l loss in mouse embryo fibroblasts (MEFs) with floxed Ash2l alleles and an inducible Cre-ER recombinase (iMEF-Ash2lfl/fl-Cre-ER). While the knockout of Ash2l was rapid, the downstream effects, including the decrease in promoter-associated H3K4me3, altered gene…

Annotate list of GSMxxxx GEO sample IDs with matching source GSExxx dataset identifier? 0 I have file of gene expression data produced by ARCHS4 which is a table of genes in rows and sample IDs (e.g., GSM1132717, GSM1132728, GSM1233280 etc.) There are ~8K columns since this was a download of…

Characteristics of potential breeding sites for Anopheles Malaria is prevalent in South Korea, with an average of 500 cases annually. COVID-19 disrupted malaria services, leading to a significant increase in cases and deaths worldwide [38]. Interestingly, the number of malaria cases in Korea decreased below 274 during the COVID-19 pandemic,…

DOI: 10.18129/B9.bioc.airway     This package is for version 3.15 of Bioconductor; for the stable, up-to-date release version, see airway. RangedSummarizedExperiment for RNA-Seq in airway smooth muscle cells, by Himes et al PLoS One 2014 Bioconductor version: 3.15 This package provides a RangedSummarizedExperiment object of read counts in genes for…

Allam RM, Al-Abd AM, Khedr A, Sharaf OA, Nofal SM, Khalifa AE, Mosli HA, Abdel-Naim AB (2018) Fingolimod interrupts the cross talk between estrogen metabolism and sphingolipid metabolism within prostate cancer cells. Toxicol Lett 291:77–85 Article  CAS  PubMed  Google Scholar  Andrews S et al (2010) FastQC: a quality control tool…

Nucleus preparation from frozen brain tissue for Chromium single-cell multiome ATAC and gene expression analysis M1 tissue was obtained from three human donors (male, aged 42, 29 and 58 years), three macaque donors (male, aged 6 (Macaca mulatta), 6 (M. mulatta) and 14 (Macaca fascicularis) years), three marmoset (Callithrix jacchus)…

‘Country’ will transition to ‘Geographic Location’ effective June 2024 As announced earlier this year, we will begin to systematically gather ‘location of collection’ and ‘date and time of collection’ for sequence data submitted to GenBank and the Sequence Read Archive (SRA). As part of this effort and to make location data more accurate and informative,…

Almarez DN, Almarez FJS, Baulete EM. 2014. Bayug Island Aquasilvi Program: An Eco-Governance Strategy for Climate Change Adaptation and Mitigation. J Govern Dev 10(2): 35-54. DOI: 10.32890/jgd. Alongi DM. 2014. Carbon cycling and storage in mangrove forests. Ann Rev Mar Sci 6: 195-219. DOI: 10.1146/annurev-marine-010213-135020. Balint M, Pfenninger M, Grossart…

Mouse genetic models The ThrbHAB allele expresses TRβ proteins (TRβ1 and TRβ2) fused to a peptide with a hemagglutinin (HAx2) tag and a site for biotinylation by prokaryotic BirA ligase, modified from a published tag30. The tag was inserted at the endogenous Thrb gene by homologous recombination in W9.5 (129/Sv)…

The recent pandemic caused worldwide health, economic, and social disruptions with its reverberations still felt today. A key takeaway from this event is the need for accurate and accessible tools for detecting and tracking infectious diseases. Timely identification is essential for early intervention, managing outbreaks, and preventing their spread. This…

Datasets To avoid the so-called ‘dataset bias’20,that some datasets are generated with specific structures and thus the results are ‘over-optimistic’ (in the case of working with our novel method), we performed the analysis in the light of several real datasets (see Table 4). We used four different datasets from the NCBI…

QUESTION 3 1.What is produced by the following code? library(nlme)library(lattice)xyplot(weight~Time|Diet,BodyWeight) Note: To answer this question, please run the above code in your R-Studio environment. A set of 3 panels showing the relationship between weight and time for each diet. A set of 16 panels showing the relationship between weight and…

Introduction With nearly 2 million new cases and 1 million deaths worldwide in 2020, colorectal cancer is the third-most common cancer and the second leading cause of cancer-related deaths.1 According to data from the US Surveillance, Epidemiology and End Results program and the National Program of Cancer Registries program, the…

Kaiyuan Zhang,1,* Ziyue Luo,1,* Xinchao Zhu,1 Xinyi Yao,1 Dingqi Lu,2 Liying Chen,1 Tao Hong,1 Yating Ren,1 Xinchang Wang3 1Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang Province, 310053, People’s Republic of China; 2First Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang Province, 310053, People’s Republic of China;…

Hello Everyone , I am new to microarray dataset . I want to do this similar kind of plotting using this same mentioned dataset for a different gene . GEO ID : GSE76008 I have tried GEO2R script : <h6>#</h6> library(GEOquery) library(limma) library(umap) gset <- getGEO(“GSE76008”, GSEMatrix =TRUE, AnnotGPL=TRUE)[1] fvarLabels(gset)…

4 Fastq files for a single run generated by 10X 0 Hello, I have a question about the 10X generated Fastq files. As I know 10X platforms can generate up to 4 Fastq files as R1, R2, I1 and I2. I need to use Fastq files and align them with…

using RMA normalized microarray in Limma 0 Hi, i downloaded raw.tar file containing .CEL microarray data from GEO database. and then i use affy() package in R to extracted .CEL file and normalized with RMA(). Can i use this expression data to put in Limma? or it is better to…

I have a very specific problem that only occurs when using the SLURM job manager on the high-performance cluster (HPC) of my university. It does not happen when I run the code on the login node of the HPC or on my own computer. When I call FDTD via Python,…

DOI: 10.18129/B9.bioc.KEGGdzPathwaysGEO   KEGG Disease Datasets from GEO Bioconductor version: Release (3.18) This is a collection of 24 data sets for which the phenotype is a disease with a corresponding pathway in the KEGG database.This collection of datasets were used as gold standard in comparing gene set analysis methods by…

Technical replicates in Microarray data 1 I am trying to analyze a microarray dataset from GEO in which one of the patients has 5 technical replicates. Should I calculate the average gene expression of 5 replicates? What is the best solution? GEO Microarray • 1.7k views The best way to…

Introduction Patients with persistent, recurrent, or metastatic (P/R/M) cervical carcinoma respond poorly to treatment despite the best available therapeutic regimens, with a 5-year survival of 17%.1 Most of them are heavily pretreated with chemotherapy and/or radiotherapy, and many patients experience complications related to treatment or advanced disease, which exclude them…

Combine Geoids 0 I have the following geo IDs: GSE230470—>Calorie Restriction Outperforms Bariatric Surgery in Reversing Obesity-Driven Tumor Progression in a Mouse Model of Triple Negative Breast Cancer-Study1_tumor GSE230471—>Calorie Restriction Outperforms Bariatric Surgery in Reversing Obesity-Driven Tumor Progression in a Mouse Model of Triple Negative Breast Cancer-Study2_tumor GSE174761—>Bariatric surgery reduces…

Image by Gerd Altmann from Pixabay For downloading data and standardized metadata from GEO (Gene Expression Omnibus) and SRA (Sequence Read Archive), several bioinformatics and command-line tools and scripts are available, primarily hosted on GitHub. ARA: An automated pipeline developed for better sampling of NCBI SRA database records, allowing full…

Method selection We reviewed the published literature through February 2020 to identify methods that met the following criteria: i. Descriptively categorized as (a) annotation-based; (b) pleiotropy-based; or (c) eQTL-based. ii. Utilized GWAS summary statistics, as opposed to individual-level genotype data. iii. Implemented using freely-available software or packages. iv. Provided either…

Selecting suitable geoid for study 0 I am working on the study “Phytosterols’ Effect on Epigenetic Mechanisms in Breast Cancer.” For this purpose, I have to select geoids. My basic purpose is to select an RNA-seq experiment. I searched the following geoids: GSE230470—>Calorie Restriction Outperforms Bariatric Surgery in Reversing Obesity-Driven…

Cea Soriano L, López-Garcia E, Schulze-Rath R, Garcia Rodríguez LA. Incidence, treatment and recurrence of endometriosis in a UK-based population analysis using data from The Health Improvement Network and the Hospital Episode Statistics database. Eur J Contracept Reprod Health Care. 2017;22(5):334–43. Article  PubMed  Google Scholar  Guo SW. Recurrence of endometriosis…

Introduction The potential role of opioids used in oncology patients has been controversial. Epidemiological and retrospective studies have demonstrated that lower opioid doses and regional anesthesia (epidural, intrathecal, or paravertebral) for breast,1 colon,2 or melanoma3 are linked to lower rates of cancer recurrence, while general anesthesia with high opioid doses…

Linear Models for Microarray Data Bioconductor version: 2.5 Data analysis, linear models and differential expression for microarray data. Author: Gordon Smyth with contributions from Matthew Ritchie, Jeremy Silver, James Wettenhall, Natalie Thorne, Mette Langaas, Egil Ferkingstad, Marcus Davy, Francois Pepin, Dongseok Choi, Di Wu, Alicia Oshlack, Carolyn de Graaf, Yifang…

Introduction Lung cancer is one of the most common types of cancer worldwide and the leading cause of cancer death.1 The main category of lung cancer is non-small cell lung cancer, accounting for about 85%, and lung adenocarcinoma, as a kind of non-small cell lung cancer, is the most frequently…

Per user feedback, we are hosting a comprehensive training on how to effectively use sample level public data and metadata from sources like GEO, SRA, TCGA, GTEx, Blueprint, CCLE and other sources through Ingenuity Pathway Analysis (IPA) and IPA Analysis Match Explorer feature. The trainer will walk through usecases in…

Introduction: To investigate the molecular alterations of cuproptosis-related genes and to construct the cuproptosis-related circRNA-miRNA-mRNA networks in neovascular age-related macular degeneration (nAMD). Methods: The transcriptional profiles of laser-induced choroid neovascularization (CNV) mouse models and nAMD patient samples were obtained from sequencing and from the GEO database (GSE146887), respectively. The expression…

Three questions about datasets 2 Hello, I have three questions about Rna-seq and datasets: Is it fine to combine datasets? Suppose I am doing a project comparing control tongue epithelial tissue vs. tumor tongue epithelial tissue through DESEQ2 analysis. I have 5 control sra files from one experiment and 5…

AbbVie’s Genomics Research Center Computational Genomics is looking for a highly motivated computational biologist (Senior Scientist II, Bioinformatics) to join a team of bioinformatics scientists investigating aging and age-related diseases. AbbVie’s GRC is a center of excellence for bioinformatics, functional genomics, and human genetics. The GRC works across all R&D…

Introduction Chronic obstructive pulmonary disease (COPD) is a common chronic bronchitis disease characterized by persistent airflow limitation, which can be prevented and treated. COPD is the third leading cause of death in the world reported in 2020.1 With the progression of the disease, COPD will lead to respiratory failure, pulmonary…

SK Geo Centric held a groundbreaking ceremony on Wednesday, Nov. 15, for the Ulsan Advanced Recycling Cluster (ARC), a waste recycling complex. The site represents a total projected investment of KRW 1.8 trillion ($1.4 billion USD) and will cover 60 acres. The Ulsan ARC is scheduled to be completed by…

Bioinformatics analysis The GEO database (www.ncbi.nlm.nih.gov/geo/) provided detailed gene expression information for the GSE62165 microarray. Differentially expressed genes were identified using the online tool Morpheus (software.broadinstitute.org/morpheus/) (fold change = normal pancreatic tissue gene expression/PDAC tissue gene expression, P value < 0.05 and |log2(fold change)|>1). The downstream analysis was based on the genes with the…

Hello Biostars, I have been stuck on an issue for quite some time now, and I hope someone can help me or point me in the right direction. I’ve received my bulk RNA fastq files and analyzed it in R using DESeq2. I have found an article that provide snRNA-seq…

RiboSplitter pipeline Figure 1 shows a flowchart of the pipeline (see “Methods” for details). The key insight in RiboSplitter is to initially go small and predict the reading frame of the first 5´ exon of the splicing event, instead of trying to translate the full isoform. This is because the full…

Mice The research conducted in this study complied with all of the relevant ethical regulations. The animal protocols were approved by and performed in accordance with the Institutional Animal Care and Use Committee of St. Jude Children’s Research Hospital. C57BL/6, OT-I50, pmel51 and Rosa26-Cas9 knock-in52 mice were purchased from The…

Introduction Chemoresistance is a major challenge for breast cancer treatment.1 The mechanisms of chemoresistance are complex because of crosstalk between receptor tyrosine kinases and downstream pathways, deregulation of cell-cycle and apoptosis regulators, and modulation of tumor-infiltrating immune cells.2 The ATP-binding cassette (ABC) superfamily is one of the largest families of…

For the Imageable Genome project, we developed a data pipeline that identifies texts containing radiotracers, recognizes and extracts names of radiotracers from texts, filters for clinically relevant radiotracers and their associated targets, and translates protein names, i.e. of radiotracer targets, to names of the coding genes. We then downloaded the…

Using GEO RNA seq datasets for retrieving gene expression and analysis in partek genomic suite 0 I am learning to use partek genomic suite to analyze GEO datasets. I am stuck at the end stage where I am not able to see any gene symbols(Coulmn 2- Column ID))in the spreadsheet…

mRNA expressions of DDR-related genes are upregulated in CRPC compared to those in Pca To identify a cluster of upregulated genes in CRPC, we previously conducted directional RNA-seq analysis using clinical samples obtained from localized Pca and CRPC patients21. We used RNA samples obtained from prostate cancer patients by radical…

St. Jude Children’s Research Hospital is looking for a highly motivated Senior Bioinformatics Analyst/Bioinformatic Analyst to contribute to genetic research projects in the Department of Epidemiology and Cancer Control. Our research leverages comprehensive biomedical data from childhood cancer survivors participating in two world-leading cohort studies, CCSS (ccss.stjude.org/ ) and SJLIFE…

I want to use CellAssign to annotate scRNA-seq data, but I faced the error and I don’t know how to solve it. Could anyone help me? This is my code: rowdata <- rownames(N_data) coldata <- colnames(N_data) sce <- SingleCellExperiment(assays = list(counts = as.matrix(N_data)),colData = coldata,rowData = rowdata) sce <- computeSumFactors(sce)…

Hello everyone, I’ve been having the same question for a while now. I’m also conducting my own analysis of differential expression on a microarray dataset in R. However, the data is different from the results obtained using GEO2R. Here’s my line of code: my_id <- “GSE80178” gse <- getGEO(my_id, GSEMatrix…

Submit Microarray Data on geo without Cell files 0 I have Affymetrix microarray data. The organism is a mouse. Total RNA was used to generate cRNA, which was labeled with biotin according to techniques recommended by the CodeLink Expression Bioarray System (Amersham, UK). I don’t have cell files; I only…

I remained for about 15 minutes spitting out and brushing termites from my clothes. While confined to the loo, I took the opportunity to reel in my Chapstick concealment device and update my report. Done, I tossed out the stick and cut the light out. I cracked open the bathroom…

In their methods they state it’s a matrix market format. And the count matrix is in MatrixMarket format. However, the file on GEO is missing the MM header. Usually the first (non %%) line will have the row number, column number, and number of non-zero values. This lets whatever reader…

The global stance, in the current scenario of the pandemic, has imposed a substantial burden on healthcare practitioners. The ultimate aim of researchers and scientists worldwide is to fight the ongoing situation and discover significant vaccines which can reduce the mortality rate among society. As we know, the decisive way…

Illumina HumanHT-12 V4.0 expression beadchip 2 I’m quite new to the bioinformatics world and just started working with the GEO data set GSE119600. My intention is to extract deferentially expressed genes and will start my work using the Lumi package to import ,read and normalize the raw data into R….

Comment: Accounting for Batch effect reduces DEGs drastically by rohitsatyam102 &utrif; 20 I might have missed out one detail which might actually be relevant now that I revisited my code. Previously using old reference genome GRC… Comment: Accounting for Batch effect reduces DEGs drastically by rohitsatyam102 &utrif; 20 I don’t…

Abstract Low-level mosaic epimutations within the BRCA1 gene promoter occur in 5–8% of healthy individuals and are associated with a significantly elevated risk of breast and ovarian cancer. Similar events may also affect other tumor suppressor genes, potentially being a significant contributor to cancer burden. While this opens a new…

Zack TI, Schumacher SE, Carter SL, Cherniack AD, Saksena G, Tabak B, Lawrence MS, Zhang C-Z, Wala J, Mermel CH. Pan-cancer patterns of somatic copy number alteration. Nat Genet. 2013;45:1134–40. Article  CAS  PubMed  PubMed Central  Google Scholar  Dixon JR, Xu J, Dileep V, Zhan Y, Song F, Le VT, Yardımcı…

Error in Gviz (actually, rtracklayer) | IdeogramTrack 0 @25075190 Last seen 7 minutes ago South Korea When I run this code (below) iTrack <- IdeogramTrack(genome = “hg19”, chromosome = “chr2”, name = “”) then I get the error Error: failed to load external entity “http://genome.ucsc.edu/FAQ/FAQreleases” Did someone else encounter this…

Downstream analysis during sc-ATAC seq 0 Hi, I have been analysisg a single-cell ATAC seq data published in a Cell Paper titled “Single-cell analyses reveal key immune cell subsets associated with response to PD-L1 blockade in triple-negative breast cancer” (DOI: 10.1016/j.ccell.2021.09.010), and I have retrieved the ATAC seq data from…

About Elucidata Delivering ML-ready biomedical molecular data. Job Description Elucidata’s mission is to power drug discovery by harnessing the power of structured and unstructured biomedical data. Our state-of-the-art technology transforms data so that it is analysis-ready. Biomedical data is vast but unstandardized, hence it remains underutilized. Or as we say…

Introduction GC is a common malignant tumor of digestive system. According to the International Agency for Research on Cancer (IARC) of the World Health Organization, there were 19.29 million new cancer cases and 9.96 million deaths worldwide in 2020, of which the number of new cases of GC was 1089,103…

Introduction Arteriosclerosis obliterans (ASO) is a chronic disorder with atherosclerosis involving lower extremity arteries leading to arterial stenosis or occlusion. The incidence continues to increase with age, affecting about 202 million people worldwide to varying degree.1–3 Despite the proposed theories of lipid infiltration, arterial intimal injury, and chronic inflammation, the…

Can I still remove ambient RNA if I do not have raw barcode-gene matrix? 0 Hi, I would like to use SoupX for ambient RNA removal. However, I am using barcode-gene matrix datasets downloaded from NCBI GEO which don’t always include raw data. Can I still use SoupX without the…

Breadcrumb Trail Links Business Wire News Releases Author of the article: Published Oct 23, 2023  •  5 minute read Article content VANCOUVER, British Columbia — Northisle Copper and Gold Inc. (TSX-V: NCX) (“Northisle” or the “Company”) is pleased to announce that the Company has commenced a Phase 3 drill program…

PASAPASA was originally developed at The Institute for Genomic Research in 2002 as an effort to automatically improve gene structures in Arabidopsis thaliana. Since then, it has been applied to numerous Eukaryotic genome annotation projects including Rice, Aspergillus species, Plasmodium falciparum, Schistosoma mansoni, Aedes aegypti, mouse, human, among others. Functions of PASA…

AbbVie’s Genomics Research Center Computational Genomics is looking for a highly motivated computational biologist (Senior Scientist II, Bioinformatics) to join a team of bioinformatics scientists investigating aging and age-related diseases. AbbVie’s GRC is a center of excellence for bioinformatics, functional genomics, and human genetics. The GRC works across all R&D…

AbbVie’s Genomics Research Center Computational Genomics is looking for a highly motivated computational biologist (Senior Scientist II, Bioinformatics) to join a team of bioinformatics scientists investigating aging and age-related diseases. AbbVie’s GRC is a center of excellence for bioinformatics, functional genomics, and human genetics. The GRC works across all R&D…

Trouble finding datasets 1 Hello, I am trying to find datasets for a project on HNSCC. I have been using GEO as my main website to find datasets but have not found anything. I am trying to find a dataset about HNSCC, tumor and control, RNA, for the tonsil body…

Background: Alzheimer’s disease (AD) occurs in the elderly and pre-elderly, characterized by decline of memory, cognitive dysfunction, impairment of learning capacity, and motor dysfunction. Recently a competitive endogenous RNA (ceRNA) network has been found to be related to AD progression, but there is still little understanding of the ceRNA regulatory…

Introduction Crohn’s Disease (CD) is a typical group of inflammatory bowel disease, a chronic intestinal disease with an unclear cause that fluctuates between clinical remission and relapse. The disease may affect the entirety of the gastrointestinal tract, frequently manifesting as segmental, asymmetric, and transmural lesions. 21–47% of patients present with…

Discrepancies Between GEO Page and its article – Seeking Clarification 0 How should I interpret the information from the article GSE145281? On the GEO page, it mentions bladder cancer. However, the article itself specifies that the samples are from both metastatic urothelial cancer and metastatic RCC. Which is the correct…

SilicoScientia Pvt Ltd is looking for a freelance scientific writer with extensive experience in Bioinformatics – Genomics as the core area of scientific research.  Responsibilities § Should be able to write scientific manuscript independently for publishing the same in peer review journal. § Should have sound knowledge for providing query based Bioinformatics…

GEO Dataset: Difficulty Understanding Matrices, Compliation 1 Hi, I am new to using GEO, novice at R (have used seurat for my own scRNA data), apologies for how basic this question is: I am trying to replicate figure 1 from a recent paper using seurat. They’ve deposited their data as…

We also are actively involved in developing new gene editing methods and provide opportunities for collaborations on more extensive or challenging uses of CRISPR techniques such as development of new construct designs, application to whole organisms, development and use of pooled genome-wide CRISPR libraries. Services/Equipment available Engineered mammalian and bacterial…

Introduction Hypertrophic cardiomyopathy (HCM), characterized by asymmetric hypertrophy of the ventricular wall, is a condition where the heart becomes thickened without a distinct inducement.1,2 Epidemiological investigation shows that the estimated prevalence rate of HCM in the general population is 1:500.3,4 The clinical manifestations vary greatly, with no symptoms and mild…

SRR download using fasterq-dump 1 Hello, I have downloaded the sra-toolkit from Anaconda (anaconda.org/bioconda/sra-tools) and downloaded an .sra file using the command: prefetch SRR20073591. The .sra file is located here: /faststorage/project/Biof/testdir/SRR20073591/SRR20073591.sra. When I navigate to the directory and use this command: fasterq-dump SRR20073591.sra, I get an output file called SRR20073591.fastq….

(1) WAYS IN WHICH PRATCHETT IS STILL WITH US. Sam Jordison discusses “Pratchett power: from lost stories to new adaptations, how the late Discworld author lives on” in the Guardian. “Of all the dead authors in the world, Terry Pratchett is the most alive,” said John Lloyd at the author’s memorial in…

guerlab-cloud-web-webmvc   Guerlab cloud web webmvc 2022-11-19T08:18:30+00:00 guerlab-cloud-web-webflux   Guerlab cloud web webflux 2022-11-19T08:18:29+00:00 guerlab-cloud-web-core   Guerlab cloud web core 2022-11-19T08:18:29+00:00 guerlab-cloud-web   Guerlab cloud web 2022-11-19T08:18:29+00:00 guerlab-cloud-stream   Guerlab cloud stream 2022-11-19T08:18:28+00:00 guerlab-cloud-server-web   Guerlab cloud server web 2022-11-19T08:18:28+00:00 guerlab-cloud-server-orm   Guerlab cloud server orm 2022-11-19T08:18:28+00:00 guerlab-cloud-server-commons   Guerlab…

. 2023 Sep 18:14:1197418. doi: 10.3389/fphar.2023.1197418. eCollection 2023. Affiliations Expand Affiliation 1 Chongqing Three Gorges Medical College, Chongqing Key Laboratory of Development and Utilization of Genuine Medicinal Materials in Three Gorges Reservoir Area, Chongqing, China. Free PMC article Item in Clipboard Wei Xiong et al. Front Pharmacol. 2023. Free PMC article…

Abstract Background: Diffuse large B-cell lymphoma (DLBCL) is the predominant type of malignant B-cell lymphoma. Although various treatments have been developed, the limited efficacy calls for more and further exploration of its characteristics. <br />Methods: Datasets from Gene Expression Omnibus (GEO) database were used for identifying the tumor purity of…

The exploration of methylated biomarkers in plasma for pancreatic cancer is still in its early stages, and the number of studies conducted to date for this purpose is limited. Analysis of cfDNA methylation patterns in pancreatic cancer has been approached both at the whole genome level and by identifying and…

Name Last modified Size Description Parent Directory   –   2anova1.jpg 2011-09-09 05:55 121K   4.3.1.jpg 2011-09-09 05:55 117K   4.4.1.jpg 2011-09-09 05:55 63K   4.6.1.jpg 2011-09-09 05:55 38K   4.7.1.jpg 2011-09-09 05:55 56K   4.10.1.jpg 2011-09-09 05:55 63K   4.11.1.jpg 2011-09-09 05:55 112K   4.12.1.jpg 2011-09-09 05:55 55K  …

PDUFA Target Action Date of June 26, 2024 Pooled analysis and subgroup data further support potential of ensifentrine, an investigational, novel, selective, dual inhibitor of PDE3 and PDE4 LONDON and RALEIGH, N.C., Oct. 03, 2023 (GLOBE NEWSWIRE) — Verona Pharma plc (Nasdaq: VRNA) (“Verona Pharma” or the “Company”), announces four presentations…

PDUFA Target Action Date of June 26, 2024 Pooled analysis and subgroup data further support potential of ensifentrine, an investigational, novel, selective, dual inhibitor of PDE3 and PDE4 LONDON and RALEIGH, N.C., Oct. 03, 2023 (GLOBE NEWSWIRE) — Verona Pharma plc (Nasdaq: VRNA) (“Verona Pharma&CloseCurlyDoubleQuote; or the “Company&CloseCurlyDoubleQuote;), announces four…

Large fold change when analyzing expression profiling from microarray 0 Hi, dear all. I am trying to analyze a expression profiling dataset from GEO, which was detected by Affymetrix microarray. Its data processing method was described as “The data were analyzed with Microarray Suite GCOS using Affymetrix default analysis settings…

Visiongain has published a new report entitled Bioreactors Market Report 2023-2033 : Forecasts by Product Type (Single-use, Reusable), by Cell (Mammalian Cells, Bacterial Cells, Yeast Cells), by Molecule (Monoclonal Antibodies, Vaccines, Recombinant Proteins, Stem Cells, Gene Therapy), by Usage (Developmental, Pre-clinical & Clinical, Commercial Production), by End-use (R&D Organisations, Biopharma…

Introduction Protein levels are regulated at several nodes. One mode of protein level regulation acts through enhancing or reducing gene transcription. Gene transcription can be stimulated by binding transcription factors to promoters or enhancers of target genes and by posttranslational modifications of transcription factors and histones. Such modifications can be…

Why are cats such picky eaters when dogs seem willing to wolf down just about anything they encounter? It’s a question many pet parents likely ask themselves while coaxing their feline friend to swallow a pill or prying another slobbery piece of mulch from their puppy’s mouth. There’s still a…

Open Access ARTICLE MENGQIN ZHU1,2,3,4,#, TIANYI XU5,#, HAN ZHANG3,4, XIN FAN3,4, YULAN WANG6, JIAJIA ZHANG3,4, FEI YU1,2,3,4,* 1 Shanghai Clinical College, Anhui Medical University, Shanghai, 200040, China2 The Fifth Clinical Medical College, Anhui Medical University, Hefei, 230032, China3 Department of Nuclear Medicine, Shanghai Tenth People’s Hospital, Tongji University School of…

Background: The presence of genetic variations in mitochondrial DNA (mtDNA) has been associated with a diverse array of diseases. The objective of this study was to examine the correlations between mtDNA D-loop, its haplotypes, and polycystic ovary syndrome (PCOS) in the Chinese population, and the associations between mtDNA D-loop and…

Source: Youtube.com The era of big data has revolutionized the way we analyze and interpret complex biological systems. In the field of genomics, Chip-Seq (Chromatin Immunoprecipitation Sequencing) data has become a valuable resource for understanding gene regulation and the functionality of the genome. Chip-Seq data provides insights into the binding…

Identification of the anti-inflammatory target of active ingredients in Morchella esculenta The active ingredient of “Morchella esculenta” according to the NPASS database was (E)-Octadec-11-En-9-Yinoic Acid (EOYA) (Fig. 1), numbered NPC179764, with a chemical formula of C18H30O2, and ‘SMILE’ value, CCCCCC/C=C/C#CCCCCCCCC(=O)O. The targets obtained by searching the “EOYA Smile Value” in the…

Cell culture All cell lines were grown in 6 cm dishes at 37 °C in a 5% CO2 incubator. The K562, MDA-MB-231 and HCT 116 cell lines were cultured in high-glucose DMEM supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin antibiotics (pen-strep). NCM460 cells were cultured in RPMI 1640 medium supplemented…

Why dbConnect GEOmetadb_demo.sqlite shows some information while dbConnect GEOmetadb.sqlite shows no information 0 sqlfile_demo <- getSQLiteFile(destdir = “~/bin/Rpack”, destfile = “GEOmetadb.sqlite_demo.gz”,type=”demo”) con <- dbConnect(SQLite(),sqlfile_demo) getBiocPlatformMap(con)[1:5,] output: title gpl bioc_package manufacturer 1 [Maize] Affymetrix Maize Genome Array GPL4032 Affymetrix 2 Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version) GPL13497…

Abstract Idiopathic pulmonary fibrosis (IPF) is a chronic progressive lung disease with reduced quality of life and earlier mortality, but its pathogenesis and key genes are still unclear. In this investigation, bioinformatics was used to deeply analyze the pathogenesis of IPF and related key genes, so as to investigate the…

In GEO NCBI repository are available single cell data on PC9 untreated lung cancer cell line done in two different labs, using both 10XGenomics and Drop-seq platforms and produced from in vitro culture or xenograph experiments. set1: GSM3972657 PC9 in vitro dropseq Chicago (2500 cells) set2: SM4494347 PC9 in vitro…

Aken BL, Ayling S, Barrell D, Clarke L, Curwen V, Fairley S et al (2016) The Ensembl gene annotation system. Database (Oxford) baw093 Bagaria J, Nho K, An SSA (2021) Importance of GWAS in finding un-targeted genetic association of sporadic Alzheimer’s disease. Mol Cell Toxicol 17(3):233–244 Article  CAS  Google Scholar …

The Pittsburgh Supercomputing Center (PSC), a joint research center of Carnegie Mellon University and the University of Pittsburgh, was established in 1986, and for over 30 years has provided university, government and industrial researchers with access to several of the most powerful systems for sophisticated computational research, communications and data…