Relative abundance of differentially abundant ASVs after DESeq2
I used DESeq2 to see which ASVs were differentially abundant between different treatments on 16S metabarcoding data. I now want to plot the relative abundance (in %) of those ASVs.
However, I am unsure which data would make to most sense to plot… should I use:
- the raw reads table transformed to %
- the rarefied reads table transformed to % (which I used for beta-diversity)
- the normalized or transformed reads count table (rlog or vst) from DESeq2
Wouldn’t the first two options represent the original data better?
I am sorry as this is probably a very basic question, I just can’t figure it out!
Thank you very much !
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