Mapping transcripts to mitogenome
I have performed de novo assembly of a plant mitogenome (non-model plant). In addition, I also perform de novo transcriptome assembly of this particular plant species. How to verify if the mitogenome assembly is correct making use of the RNA-Seq data?
- Should I align my sequences reads (reads) or assembled sequences (unigenes) to the mitogenome?
- What is the software recommended? MUMmer? GMAP or STAR?
• 8 views