Signac CallPeaks from multiple fragment files

Signac CallPeaks from multiple fragment files

0

I am attempting to run Macs2 CallPeaks on some multiome data and running into a problem when attempting to run CallPeaks command on multiple fragment file paths in Seurat object.

peaks<-CallPeaks(DataCombined, macs2.path = "/anaconda3/bin/macs2")

FileNotFoundError: [Errno 2] No such file or directory: '/Users/Desktop/multiome/sc291/atac_fragments.tsv.gz /Users/Desktop/multiome/sc292/atac_fragments.tsv.gz /Users/Desktop/multiome/sc293/atac_fragments.tsv.gz'

So basically its listing all three paths together as a single path, not sure how to resolve this. TIA


Seurat


Macs2


Signac

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