interpreting heatmap on metascape
thanks for adding me to the group.
I am looking for some advice on heatmap interpretation in metascape.
I am analysing two gene lists mygenelist=20 genes and another gene list of 2000 genes in metascape.
I am looking at the analysis report and my heatmap and to look at firstly one enrichment term hsa04060 which is grey for the large 2000 gene dataset and brown/orange for mygenelist a 20 gene dataset. but when i go to excel spreadsheet the term in the enrichment headings hsa04060 which is enriched for both genes RORC and CCL18 (which are also shared by both dataset lists) so why doesn’t the eczemegenelist column appear orange in the heatmap? see screenshot below:
is it that there aren’t enough genes which are shared between the datasets for it to appear orange in both columns?
I have attached a screenshot from both heatmap and the excel spreadsheet the genes I mention are highlighted in yellow on the excel sheet and there are blue arrows pointing at the relevant area on the heatmap.
I do not have direct access to any bioinformaticians and so have not got much advice on this and much appreciate any suggestions or help.
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