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Open Source Biology & Genetics Interest Group

Open source tools and preprints for in vitro biology, genetics, bioinformatics, crispr, and other biotech applications.

 Posted in Research

MACS2 BDGCMP

 August 14, 2021

MACS2 bdgcmp for two bedgraph peak callingKeep it as 1.0 or default in most … bdgcmp Deduct noise by comparing two signal tracks in bedGraph.

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Tagged bdgcmp, BED, bedGraph, MACS

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YouTube Video VVVxYU1TUWRfaC0yRURHc1U2V0RpWDBRLjhwZ2JxNWJRaHh3
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YouTube Video VVVxYU1TUWRfaC0yRURHc1U2V0RpWDBRLmctUldCSHFLajZN
Rにおけるバイオ画像解析入門: Introduction to Bioimaging Analysis in R 久米 慧嗣 Satoshi Kume (大阪電気通信大学 Osaka Electro-Communication University, 日本 Japan) 4:00 PM - 5:00 PM JST (Japan Standard Time) on Thursday, Nov. 4th, 2021 JAPANESE WORKSHOP このワークショップでは、BioconductorパッケージであるEBImageを使って、Rでの画像処理・画像解析の基本的な方法を扱う。次に、BioImageDbsパッケージを用いて、ExperimentHubからの画像データの取得を行う。さらに、rMiWパッケージが提供する、Deep learningモデルの１つであるU-NETモデルを用いて、教師有り画像セグメンテーション（領域分割）を学び。このワークショップは、Orchestra環境にて実施する。 This workshop covers basic methods of the image processing and image analysis in R using the Bioconductor package “EBImage” and the Orchestra platform. In addition, the image dataset is obtained from ExperimentHub using the “BioImageDbs” package. Using this dataset, we perform a supervised image segmentation using the U-NET model, one of deep learning models, provided by the rMiW package. Moderator: 露崎 弘毅 Koki Tsuyuzaki (理化学研究所 RIKEN, 日本 Japan)
Rにおけるバイオ画像解析入門: Introduction to Bioimaging Analysis in R
YouTube Video VVVxYU1TUWRfaC0yRURHc1U2V0RpWDBRLlhXamZHelhsRTVr
BioC Asia 2021 Opening Remark Koki Tsuyuzaki (RIKEN, Japan) 1:00 PM - 1:10 PM JST (Japan Standard Time) on Monday, Nov. 1st, 2021 OPENING REMARK
BioC Asia 2021 Opening Remark
YouTube Video VVVxYU1TUWRfaC0yRURHc1U2V0RpWDBRLnE0NHYwR0lFVW53
Dimension Reduction for Beginners: Hitchhiker’s Guide to Matrix Factorization and PCA Aedin Culhane (University of Limerick, Ireland) 4:00 PM - 5:00 PM JST (Japan Standard Time) on Tuesday, Nov. 2nd, 2021 ENGLISH WORKSHOP This workshop will provide a beginner’s guide to matrix factorization, principal component analysis (PCA), the difference between singular value decomposition, different forms of PCA and fast PCA for single-cell data as well as correspondence analysis and decomposition of the Pearson Residuals. We will describe how to detect artifacts and select the optimal number of components. It will focus on SVD, PCA, COA applied toy datasets and single-cell data. Principal component analysis (PCA) is a key step in many bioinformatics pipelines. In this interactive session we will take a deep dive into the various implementations of singular value decomposition (SVD) and principal component analysis (PCA) to clarify the relationship between these methods, and to demonstrate the equivalencies and contrasts between these methods. We will describe correspondence analysis (COA) and demonstrate how it differs from PCA. We will also discuss interpretation of outputs, as well as some common pitfalls and sources of confusion in utilizing these methods. Moderator: Peter Hickey (Walter and Eliza Hall Institute of Medical Research)
Dimension Reduction for Beginners: Hitchhiker's Guide to Matrix Factorization and PCA
YouTube Video VVVxYU1TUWRfaC0yRURHc1U2V0RpWDBRLlAwWGx4MXR4SmFz
Single-cell proteomics data analysis using QFeatures and scp Christophe Vanderaa (UCLouvain, Belgium) Laurent Gatto (UCLouvain, Belgium) 4:00 PM - 5:00 PM JST (Japan Standard Time) on Thursday, Nov. 4th, 2021 ENGLISH WORKSHOP Mass spectrometry (MS)-based single-cell proteomics (SCP) is an emerging field that requires a dedicated computational environment. QFeatures along with its extension scp allow for standardized analysis of SCP data. The workshop will start by introducing the QFeatures class and its functions to perform generic proteomics data analysis. We will then move to SCP and present how scp extends QFeatures to single-cell applications. The remainder of the workshop will be a hands-on session where attendees will be guided through the reproduction of a real-life analysis of published SCP data. Along the reproduction exercise, we will point out to current challenges that still need to be tackled computationally. This workshop is meant for inexperienced users that want to learn how to perform current state-of-the-art analysis of SCP data as well as experienced developers interested in contributing to an emerging and exciting single-cell technology. This workshop is provided as two vignettes. The first vignette provides a general introduction to the QFeatures class in the general context of MS-based proteomics data manipulation. The second vignette focuses on single-cell application and introduces the scp package as an extension of QFeatures. This second vignette also provides exercises that give the attendee the opportunity to apply the learned concepts to reproduce a published analysis on a subset of a real data set. Moderator: Peter Hickey (Walter and Eliza Hall Institute of Medical Research)
Single-cell proteomics data analysis using QFeatures and scp
YouTube Video VVVxYU1TUWRfaC0yRURHc1U2V0RpWDBRLkNMWnhqSUtpa0lV
Visualize Data on Spirals Zuguang Gu (German Cancer Research Center, Germany) 3:30 PM - 3:55 PM JST (Japan Standard Time) on Thursday, Nov. 4th, 2021 CONTRIBUTED TALK The spiral layout has two major advantages for data visualization. First, it is able to visualize data with long axes, which greatly improves the resolution of visualization. Second, it is efficient for time series data to reveal periodic patterns. Here we present the R package spiralize that provides a general solution for visualizing data on spirals. spiralize implements numerous graphics functions so that self-defined high-level graphics can be easily implemented by users. The spiralize package is available at https://CRAN.R-project.org/package=spiralize. Moderator: Kozo Nishida (RIKEN)
Visualize Data on Spirals
YouTube Video VVVxYU1TUWRfaC0yRURHc1U2V0RpWDBRLm8tUHh2Qm5rWW9J
Visualization and exploration of MSAs and associated data with ggmsa 周烺 Lang Chau (Southern Medical University, China) 4:00 PM - 5:00 PM JST (Japan Standard Time) on Thursday, Nov. 4th, 2021 MANDARIN WORKSHOP 在多序列比对(Multiple Sequence Alignment, MSA)的可视化中，普遍采用堆叠可视化的形式，其以行表示每条序列，以列表示相同位置的不同残基字符。我们开发的R包ggmsa在可视化层面扩展了这种堆叠形式的可视化方法，使得MSA图形可以与各类数据进行整合。例如：允许将序列二级结构信息、基因座位点信息或表型数据等序列相关的数据集与MSA结合，以此来探索序列-结构-功能三者之间的关系。并且，我们还开发和整合多种特别的比对序列可视化方法，包核苷酸差异图、核苷酸相似性图 (鉴定序列重组信号)、序列集束(Sequence Bundles)和序列标识(Sequence Logos)，这允许用户从不同角度探索序列的特征。ggmsa可以在git:上找到 https://github.com/YuLab-SMU/ggmsa Workshop将分为三个部分: 使用图形语法对MSA进行可视化和注释。 整合MSA图形和其他数据。 通过其他序列可视化方法探索序列特征。 The stacked alignment graphic that represents all individual sequences as rows and homologous residue positions as columns is used almost universally in the visualization of multiple sequence alignment (MSA). We present the R package ggmsa to extend MSA visualization method via integrating stacked MSAs and associated data. Sequence-related data sets such as secondary structures, genes locus, or phenotype are allowed to combining with MSA for exploring the sequence-structure-function relationship. And we developed and integrated multiple alignment visualization methods such as nucleotide difference plots, nucleotide similarity plots, and sequence bundles to exploring multifaceted sequence features from multiple perspectives. The ggmsa package is available at https://github.com/YuLab-SMU/ggmsa. The workshop will be organized into three broad sections: Visualization of MSAs and annotations via Grammar of Graphics Integration of stacked MSAs plot and associated data. Exploration of sequence features via different sequence visualization methods Moderator: Xueyi Dong (Walter and Eliza Hall Institute of Medical Research)
Visualization and exploration of MSAs and associated data with ggmsa
YouTube Video VVVxYU1TUWRfaC0yRURHc1U2V0RpWDBRLmtRYXVQcXZ3czJr
FANTOM project and data resource for understanding the transcription in the mammalian genomes Takeya Kasukawa (RIKEN, Japan) 1:00 PM - 1:40 PM JST (Japan Standard Time) on Thursday, Nov. 4th, 2021 KEYNOTE Takeya Kasukawa is the team leader of RIKEN Center for Integrative Medical Sciences, Laboratory for Large-Scale Biomedical Data Technology. His major is the development of data management technology that will lead to new discoveries in the field of biomedical science. He has maintained the database of the international collaborative research “FANTOM (Functional ANnoTation of Mammalian Genome) Project”. This database is widely provided to the research community and is expected to contribute to the elucidation of biological phenomena. Moderator: Kozo Nishida (RIKEN)
FANTOM project and data resource for understanding the transcription in the mammalian genomes
YouTube Video VVVxYU1TUWRfaC0yRURHc1U2V0RpWDBRLkxMSVF3VmxHNzZF
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