How do I match my transcript ID’s from NCBI to the corresponding gene ID’s to enable tximport into R?
New to RNA-Seq analysis and I tried to find an answer to this elsewhere. I have performed salmon alignment on my pair-end fastq files which has generated the quant.sf files for all my samples. I now need to import into R with tximport which requires a dataframe with transcript ID’s and the corresponding gene ID’s. My transcriptomic .fasta reference file is from NCBI which does not contain the gene ID’s, only the NM_transcript identifiers.
How do I find the gene ID’s I need, and in the correct order? The reference transcriptome I have been working from is Thoroughbred racehorse (EquCab3.0).
Thanks in advance!
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