MiRBase miRNA analysis with STAR
I am using the latest mice reference genome (GRCm39) for small RNAseq/miRNA-seq analysis. MiRBase database doesn’t have any GFF/GTF file for the mouse mature-miRNA/loop-miRNA.
I just have mature-miRNA and loop-miRNA fasta sequences from MiRBase. How I can use the STAR tool to generate genome index and GeneCounts by using fasta miRNA fasta file? I want use STAR for generating read counts for each miRNA by using –quantMode TranscriptomeSAM GeneCounts.
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