Spatial analysis with colocalization
I have a problem how to tackle my recent imaging task. I have about > 100 fluorescence images with 2 signals looking like this:
I wrote a programm which extracts the x- und y-position of every red and blue signal und now I want to check if there is some kind of colocalization. Does anyone has an idea how to do this? I searched for lots of papers and programs but they use pixel intensity and not x/y-coordinates for calculation. How do I visualize colocalization (i.e. which kind of diagram/ graph…)? I would be glad for every answer!
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