How does miRdeep2 normalise sequences
Hi, I have mirdeep2 output that looks like this.
#miRNA read_count precursor total seq seq(norm) mmu-let-7a-5p 43271 mmu-let-7a-1 43271 43271 7658.26 mmu-let-7a-1-3p 784 mmu-let-7a-1 784 784 138.76 mmu-let-7a-5p 43224 mmu-let-7a-2 43224 43224 7649.94
I think using the seq(norm) would be appropriate, but I cannot find how the sequences have been normalised. I think it is something like TPM but am not sure. Does anyone know?
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