Novel Approaches to Marker Gene Representation Learning Using Trained Tokenizers and Jointly Trained Transformer Models


Next-generation DNA sequencing technologies have made marker-gene DNA sequence data widely available. Analysis of microbiome data has many challenges, including sparsity, high cardinality, and intra-study dependencies during feature engineering. Language-modelling techniques may provide the means to overcome these challenges. The first step in sequence modelling is dividing the sequence into sensible tokens. We show that trained tokenization strategies, byte-pair encoding and unigram language modelling can replace traditional sliding-window based segmentation techniques for DNA marker genes in classification, clustering, and language-modelling tasks. We then propose a novel approach for feature representation of DNA marker genes, proposing a training scheme to learn dense vector representations of DNA sequences using transformer language models optimized using DNA sequence pair-wise alignment scores. We demonstrate that our representations match or exceed previously published approaches for treatment of individual marker genes and of microbiome samples while providing fixed-length, low-cardinality representations of each.

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