Initu extraction and detection of DNA using nanopores

Extraction and detection of single-molecule DNA from cells using 3D integrated nanopores. Credit: Makusu Tsutsui et al.

The ability to detect DNA from a single cell is important for the detection of diseases and hereditary diseases. Measurement of a single DNA molecule has long been possible. However, the sample cannot be detected directly at the time of extraction and no subsequent steps are required. Currently, researchers at Sanken, Osaka University are demonstrating a method for releasing DNA at measurement points.Their findings are published in Small way..

Nanopore is very Small hole It can be found in biology or designed for your purposes. There have been exciting advances in using Nanopore as a gateway. molecule Go through one by one. For example, individual DNA bases that pass through the pores are identified, enabling whole-genome sequencing.

However, despite these notable steps in single molecule detection, there was no way to ensure that the molecule reached the measurement hole, so the concentration of the DNA sample had to be increased for the measurement to be successful.

Researchers have created a 3D integrated nanopore that can rupture cells just before measurement. The released molecules are efficiently sent to the detection zone and measured without any additional steps that can cause errors.

“Our sensor has two important parts. The first is a layer that contains a large number of holes that are much smaller than the cell. Electrostatic field Is used to rupture cells, and certain substances released can pass through holes, but large debris cannot, essentially providing a filter, “says Maku Tsutsui, lead author of the study. Explains. The membrane on which the measurement is made. “

When a voltage is applied, an electric current flows through the pores due to salt ions in the surrounding solution.When large DNA molecules are also passing through the pores, this current is partially blocked and the change is Large molecule.. For example, whether the molecule (which can be in millimeters in length) is collapsed.

“Filtering effect of 3D integration Nanopore Tomoji Kawai, the corresponding author of the research, prevents obstruction of the measuring hole and makes it robust to use. “Therefore, we expect it to be used in new technologies for the rapid detection of mutant viruses at the genomic level,” he said.

Development of better nanopore technology

For more information:
Maku Tsutsui et al., Detection of intracellular single-molecule deoxyribonucleic acid using three-dimensionally integrated nanopores, Small way (2021). DOI: 10.1002 / smtd.202100542

Provided by
Osaka University

Quote: Https: // In situ extraction of DNA using nanopores (August 24, 2021) obtained on August 24, 2021 and detection

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