Microarray data normalization

Microarray data normalization

1

Dear all,

Sorry for such a basic question, but I got confused. As far as I know, the RMA method implemented in affy package is a standard method for microarray data normalization. Could you let me know if the main code data.rma.norm = rma(raw.data) normalize data in three stages, background correction, quantile normalization, and log2 transformation? So the output of the above code is log2 expression value, yes? or there is another story?

Many thanks


RMA


microarray


normalization

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