Hybrid assembly SPAdes (Ox. Nanopore + Illumina)

Hybrid assembly SPAdes (Ox. Nanopore + Illumina)

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Hi all,

I have both Oxford Nanopore and Illumina sequencing data of a bacterial genome. I would like to perform an hybrid assembly combining the two sequencing techniques.

Is SPAdes the most appropriate software to do it?

And, I typed the following command line, is it appropriate for this hybrid assembly?

spades.py -1 R1_001.fastq -2 R2_001.fastq –nanopore
combined_files.fastq -o Hybrid_assembly_output

Note that I combined into a single file all the .fastq files obtained from Ox. Nanopore sequencing.

Thank you very much for your advices/help!

Audrey


assembly


hybrid


SPAdes

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