We are currently using the Applied biosciences StepOne qPCR system. The software does not give us the SD, SEM, P-value for the expression profile.
Is there any package in R, which can do data analysis with all the statistics involved? Please do let me know.
Hi, I processed a lot of data from this instrument many years ago. You, technically, just need Ct values, from which you can calculate delta delta Ct (ddCt) ad fold-change. If you employ a non-parametric test, that can get p-values for you.