How to analyze the generated VCF file, what to do if you have multiple VCF file for the same gene?

How to analyze the generated VCF file, what to do if you have multiple VCF file for the same gene?

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I have given 40 tumor samples to NGS for the analysis and I gave them a list of specific genes only do the sequencing for lets call that gene x1, x2, x3.
Now they gave me the data in fastq format like sample1.fastq, sample2.fastq….
Now I want to check for mutations/SNPs in my gene x2.

I went through some online tutorials and did the following
Aligned my sample1.fastq to x1.fasta
and created a VCF file

Now I thinking to creat the same VCF file for all the samples and merge them.
What analysis should I do next?

VCF, sequence, BAM, SAM, Linux, bwa, Bowtie2, SAM, tools, Adapter, Removal, cancer


bwa


vcf


BAM


SAM


sequence

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