Posted in Europe, Research

Sequencing analysis of exons 5 and 6 in RUNX2 in non-syndromic patients with supernumerary tooth in Kelantan, Malaysia

 August 30, 2021



doi: 10.1007/s00784-021-04098-x.


Online ahead of print.

Affiliations

Item in Clipboard

Suhailiza Saharudin et al.


Clin Oral Investig.


.

Abstract


Objective:

The aim of this study is to do a sequencing analysis of RUNX2 in non-syndromic patients with supernumerary tooth.


Materials and methods:

Fifty-three patients with supernumerary tooth were identified retrospectively from 1,275 radiographic reviews who attended the Hospital Universiti Sains Malaysia (USM) Dental Clinic. Informed consent was obtained from the patients prior to the study. Blood samples were collected from 41 patients and DNA extractions were performed out of which 10 samples were chosen randomly for PCR amplification using designated primers for RUNX2 followed by DNA sequencing analysis.


Results:

This study involved 28 male patients (68.3%) and 13 female patients (31.7%) with a gender ratio of 2.2:1 and mean age of 15.9 ± 6.2 years. DNA extraction yielded ~ 40 ng/μl of concentrated DNA, and each DNA sample had more than 1500 bp of DNA length. The purity ranged between 1.8 and 2.0. DNA sequencing analysis did not reveal any mutations in exons 5 and 6 of RUNX2.


Conclusion:

This study did not reveal any mutations in exons 5 and 6 of RUNX2 in non-syndromic patients with supernumerary tooth.


Clinical relevance:

Analysis of mutations in RUNX2 is important to enhance the understanding of tooth development in humans.


Keywords:

Non-syndromic. RUNX2 . Signaling molecules. Supernumerary tooth.

References

    1. Scheiner MA, Sampson WJ (1997) Supernumerary teeth: a review of the literature and four case reports. Aust Dent J 42(3):160–165

      –

      PubMed

      –

      DOI

      –

      PMC

    1. Rajab LD, Hamdan MA (2002) Supernumerary teeth: review of the literature and a survey of 152 cases. Int J Paediatr Dent 12(4):244–254

      –

      PubMed

      –

      DOI

      –

      PMC

    1. Yusof WZ (1990) Non-syndrome multiple supernumerary teeth: literature review. J Can Dent Assoc 56(2):147–149

      –

      PubMed

      –

      PMC

    1. Bodin I, Julin P, Thomsson M (1978) Frequency and distribution of supernumerary teeth among 21609 patients. Dentomaxillofac Radiol 7(1):15–17

      –

      PubMed

      –

      DOI

      –

      PMC

    1. Fleming PS, Xavier GM, DiBiase AT, Cobourne MT (2010) Revisiting the supernumerary: the epidemiological and molecular basis of extra teeth. Br Dent J 208(1):25–30

      –

      PubMed

      –

      DOI

Read more here: Source link