normalising Biological replicates for differential analysis

@f4d593ce

Last seen 1 day ago

I am new to DESEQ2 and RNA quantification and analysis in R. I have been given a dataset of 60k rows (of genes) and16 columns for 4 treatment conditions comprising of 4 repeat cell sample each. I am just confused to import the data into R and hoe to normalise the biological replicates to perform differential analysis of gene expression between these conditions?
“`