Can the Non strand specify RNAseq data annotate genome?

Can the Non strand specify RNAseq data annotate genome?

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Hi everyone,

I have many rnaseq samples data, but I find those data are not strand specific. I read many paper and I found they annotated their genome using the strand specific RNAseq data. Can I use my non-strand specify RNAseq data to annotate gene structure of genome?Thank you very much.


RNA-Seq

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