DESeq2 Not full Rank – Age & Medication

DESeq2 Not full Rank – Age & Medication

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Hi. I’ve a data matrix which looks like this:

Metadata

I’m trying to carrying out DGE analysis controlling for age and medication so I used the following:

design(ddsMF)<-formula(~age+medication+group)
ddsMF<-DESeq(ddsMF)

And end up with the error, “Full model matrix is less than full rank.”

I saw the vignettes on the DESeq2 tutorial but am not sure if they would work here given the large age range. Any advice would be most welcome.


medication


DESeq2


age


rank

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