Why Ks value is NA in KaKs calculator

Why Ks value is NA in KaKs calculator

1

My sequence is :

>AAA
ATGGTGGGGCAGACCATGATGCGCATCGTGCGGCCCTGCTTCAAGCCCTCGCTGCCCGACGGCGCGCAGGTCGTCGCCGCCGGGGGCGGCGGCACCAGGGAGGGCCTGCTCTGGTACAGGGACGCCGGCCGCCACGCCTGCGGCGACTTCTCCATGGCCGTCGTGCAGGCCAACCAGCTGCTCGAGGACGCCAGCCAGCTCGAGGCCGGGCCCCTCGTCGCCGCCGACGGGCCCTGCGCCACCTTCGTCGGCGTCTACGACGGCCACGGCGGGCCCGAGACCGCCCGCTTCGTCGCCGATAACCTCTTCCACCACCTCAAGAAGTTTGCGACGGAACAACAGACAGTCTCGGCCGACGTGATACGCCGATCCTACGCTGCCACGGAGGAGGGCTTTCTGAACCTTGTGAGGAAGCAGTGGCTCATCAAGCCGCAGATCGCCTCGGTCGGTACATGTTGTTTGGTTGGCATTATCAACGAAGGTGTTCTGTACATAGCAAACGCCGGCGACTCCCGTGCTGTCCTCGGGAGAGTCGAGCGGGGCGCCAAAGACATTAAGGCAGTTCAGCTCTCGTCCGAGCATAACGCGAGCTTTCAGGAGGTAAGGGATGAGCTAAGACAGATGCACCCGGACGACCCCCGGATCGTGGTCCTCAAGCACAATGTTTGGCGCGTCAAGGGCATTATTCAGGTTTCCAGAACGATCGGTGACGCCTACCTAAAAAGTTCGGAGTTTAACCGCGAGCCTCTTCTGGCCCGGTTCCGTATTCCAGGGCCCTTCCATAAACCGATTCTTTGTCCGGAACCATCCATAGAAGAACACAGACTGTGCGCAGAAGATCAGTTTGTTATATTTGCGTCAGATGGACTATGGGAGCACTTGAGCAATCAGGAAGCTGTGGATATAGTTCATTGTTCGCCTCGGAATGGCATTGCGAGACGACTAATAAAAGCAGCTCTGCGGGAGGCGGCAAAGAAGAGAGAAATGAGATACTCAGACTTGAAGAAGATCGACCGCGGGGTCAGGAGGCACTTCCACGATGATATTACTGTTGTGGTATTATTCATGGACCCTGCGCTTGTCAGCAGGAGGCTCTACGGTGGGCCGTTGCTTTCGCTAAGGGGTGGTGGCAGCACGCCAACGTTTGCACAGAAATGC
ATGAATGACACTAATCCCTCGGTTCCATCTTTCAGGAGGCAGATTCTGCTCGGTCTCTCTTGGTTTAGGCCAACGCAGATGGTTGATCCTGACGATCTTCGGAGGGCGATCGGTCTTCCTGTTACTGCTTCGCTGGGCCCTGTTGGTCCCCTCCTCCGGCAACTCCATGAGGGGGTCAGTGAGGATGAGATTCAGAGGCTCACGGTGCATTTCGATGGATTATGCGTCTCCCTCATGCATCAGTCAGAGGTGGAGAAACCTAGCTTCGCCGCCAAGTGCTGGATGAGTGAGGTCCGGGAACTTTGCTACGACAGCAAAAACTACTTCGACGATGCCCCGTTTATTTCCAAGCAAGACGACGACGATGATGATGCCCAGCTCACTCCAGAAGAGAAGCAAGAAAATGCCCAGTTCGAGTTCAAGATGATCGAGGCTCGCGCGGAGCATGCGTATGGGAGAAGGGGAAGGTACGGATTCGACCAGAAAAGCGTCAATAAGACCACTAAGATTCCATCAACAGGACGCCGGCTCCCAGTTCTCCATCCGGAACTAATTGATCGTTGCCGAATGGATCAACTTGACACCTTGCTTCGTAAGGATGAGGAGCAGCAGCTCAAGGTCGTATCTCTGGTAGGATTTGAGGGTGTCGGTAAAACCACACTTGCCAAGAGGTTATACCACCATAGAGGTGGGCAGTTCAACTGCCGGGCTTTTGTGCGGGTGTCTCCCAACCCGGACATGAGGAGGCTTCTAACCACCATATTCACCCAGATTCAATTGCCGACCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTGGTACTGTCTCTCAGCCTGCTTGCTGCTTGGATGCGCAGGCCCTCATCATAAATATTGCAAAATATCTCCAATCAAGAAGGTACTTAATCATAATTGATGACTTATGGAGTACTTCAGCATGGGATGTTATTAGGCGTGCTTTTCCTGATGGTGATTGTCACAGCAGAATTATAACAACCACCCAATTCGGGGATGTAGCATCGGCATGTTGTAGTTATCAGTCAAAGTACGTATTTCATATGGAACCTCTAAAATATGATCAATCACGAAAATTGTTTTTCAGTATAGTGTTTGGCTCTGAAGATAAAGTCAGTTTTCCTGAGGATTTCAGAGAAGTCTCATCTGAGATTGTCAAGAAATGTGGTGGTTTACCATTATCAATTATAAATATAGCCAGCTTGTTGCCAACAGAAGAAAGCGAAATAAACCTCCAGCAATGGATGGACATTCGAGATTATATGCCTTCCACTATGAGGACAAATCCTACGCCCGAAGGGATAAAACAAGTTCTAAACCTTGTCTACAATAATCTTTCCCCTGAACTGAAGACATGCTTGCTGTATCTTGTTGTGAATCCAGAGGGCTACATGGTGAGTAAGGATGATTTGGTGAAGCAATGGGTAATTGAAGGTTTTATACGGGAAGGACAAAACAAAGAGGAAATTGCAGGGGGTTATTTTGATGAGCTTGTCAGGAGAGGCATGATCCAGGCTGTAGACATCAATTACCGAGGGGTGCTGTCATGTACACTTCACCACATGGTACATGATCTTATTGTGAAGAAATCCATTGAGGAAAATTTCATAGTTATCCAGGACTGTTTTCAATCAGCTATAGGTCTTCCTGACAAAGTTCGTCGTCTGTCTGTCCAATTCGGTGGTGCAAGGAGCGCAAATATACCGATGGGAGTCATGATGTCTGAAGTTCGATCACTTGCATTTTTTGGATTCTATGGATGCACGGACTTCATTGAGGAGTGTAAGCTTCTTCGAGTTCTAATTCTCCATGTGTGGGCTGATGAAGATAAAGTGCCTTTTGACCTCTCTGAAATTATTAGTAAACTACTTCCATTGAGATATGTGAAGATTGCATGTAATATCGCTGTCAAACTTCCAGCCGAGATTCAAGTTCTGCAATTCCTGGAGATACTGGAAGTGGATACACCAGTAGTTGCTATCCCATCGGATATTGGAGGACTCAGCAGGCTGTCCAGTCTAAAGATTGCAGTTACAGAGCTATTGAGGGAAGACATTTGTATCCTCCAAAAATTGGCAGCCCTCACTACTCTTTCAGTGGACGTGAAGACATCACCCACTGAATGGATCGTCTTTGGAAAGGGAGGATTCTTGGTCCTCACTTTCTTCACATTCAGGTGCAGTGCACCTCGTCTGCTAATTGAAGATAGTGCAATACCTAAATGCGAGAAGCTACACCTATGTTTAAATGGCCACGAAGCATCTCCTCGAGATGGTAAGAAGCCTATCACCATCGAGCACTTGGCAAGCCTTAAAGAGATATCTGTAAAAATTTGGGGTGCAGTTGCTAACAAGGGGTCCGCCTTGACGAATGTTATTACCAATGATGCAAGAAATCCTATTCCTAACGTTCAATTGATGGGTGATACCAGTGGTGTAGAAGTCCACATGATCTTCGACCTCAACGATGGTGATTATGCTGAATTCATCCGCCTCCTTATTGGCGTGCTCGTGAGGTACGACGACGGTGAACACATCCAAGGGTATCCTCCTGTTCTGGGTAAGCAACTGTTCCCGAAAGAGCCAAACAGGTGGATGCGCATCAAGCTAGTGGCCGGCATGACTGAAACAATCCTTGCTGTTCGGGATGACAATCTGAACCTCATTGGCTTCCAGGCTAAAAACGGAAGGTGGTACGAGTTGGGGTTCGCAGGGAGGAGCACACGAATGCTTCCTGGATCCACCTTTTTAGACTGCGATTGTGACTATACAGACTTGCTACAGGTAAAAGCTACCTGTCTGGAAGAAAGAAACCGACTAGTCAGGGAAGAAATGGTGAAGATAGAGCTAGGCAAGACCTCCTCAATTGAAGCCGTGGGTATTCTCTCGGGCTACAATAATAAAACGGAAGGTGTGGCAGCTGATCGAGACACCAGGCGGGCACTGGTGCGCCTAATGCTCATGTTGTGCGAGGCCACGAGGATGACCCCATTCTTTGACACCGTGCTGGCTAGCTGGAGCGACACACAAGGAGGGAATAGCCATATCTTGAGAGAGGACCAGGCAATGTACGTTTGGTACTGGGGAAAAATGTCGGCCGTGCTGCTGGAGTGGGAGGCAACAGGTTATAAGAGTTGGGGACCAAATGAGACCACCGCGATGCTGGCCAATGAAATCAATGTCAAATCTGCACAAGAGGCACTCGCTGTCGTCAAGGTGCTTCTCAACACACCCGTCGCCCCCAACAACACTTGCACCAGAGGAGGCCGACCTCTTGTGGAGGTGTTTACCATGCGTGCTGACTCCGGTGTTGTTGACACCATTTCGGTTTTTGACGGGAGACGTGGCCAGACCATATACAGACACCATGCTAGCCCCACTCCTATCCACCACTTTTGGTCTTCGTCTCAAGGTCCTAACGATTTGGTACTCACTGGACCATATAGAGCCATCTCAGCGGAGGGCAGCTTCGCCATCCAAGTCGACAGCATCATCTCCAGGACAGACGCCTCTGACGAACCTAGTAAAGTCAGTGCTGACATGTCCTGGGATTGCTACTCCGGTGAAGCCTCCATGTACAACAAGGTCTTGACCAACCGCATCGCCACAGACGACAAAGGCCAACTTGCAGAAGTGGCCTATGCTGTGTTCAGTGACGCCGTGGAGGTCACCGTCCAAATAAAGGTGACTAGAGTTGACCCCGCCGACGTTTACGGTAAAATCACCGCGCACAACCAGCACTACGGCAGCAACAGCATACTGCTCTTCAGCCGTACAGATTGGGATTTCCTTGCAACAAAGGAGGAAAAGAGGCAAGGAAGAAGGCGTGCAAAACTGAGGGCAAGTGGAACTTCCAATTGCTGGATTCCACTTACGCGATCCGTCGTTGCGGTGCCATTAGGCTCGCCGCTTGAGATAAGGGTCAATTTGTATGCATGTCCATCCGAATATGGGCTTACTGATTATTTCAAGGAGGATATAGATTTTTCTCTCATAGATCAGCATGTGGGGCAAATCAAAAGGCACCATGTGGTGGTTCGAGTGACCTCGCTGGACATCTGCACTCTCCCGGAACAAAGTCACACAACACTGCCTACGCTCGCCATGACCTGGGTTCCAGGGGTGACAAGTATGAGGACGGGGGCAGGAATGCCCATCGAGCAGCCTGCACATGTGTATATGGACTTCAACTGTGGCAGTGGTAAGTACAGTGCATTCATGGACGACTTGTGTGAAAAGCTTGCGGAGCACCCGGGCAAAGCTGCTGTCCATGGGCGGCCTGTCCTCGCCAGGCAGCGGCGTGGAAAGCCAACGAGGCTGTTTATGATCAGGCTAGCGGGTGACAGAGATTATGAATGGGTAACTCTGGCCGTTCGGGACGACAACTTGTGCCTCGTTGGCTTCCAAACTCCGGACGGGAGGTGGCACGAGTTCGGCTCCGGCTCCATAACTTGTCTCCAAGAGTCCACCTTTTTAGGGTCCAATGGAAGCTATGATAGCTTATTAGGTGAATCTGGAGACCGGTGGCAAGAGCTGAAGCTGGGCAGGAAGTCCGCAACAGATGCCATAGGAAAGCTCTCGGCGTACGAGGACCGAGTGGGCGTAGTAGTTGATCAAGACACCAAAGTGGATTTGGCTCGCCTGATGGTCATGATATGCGAGGCCGCACGGTTGGCAGAAATCTACGACATGGTGAAGAAAGGATGGGAAGACGGAATCTGTATCACCGACCGGCAGGTACAGCACATAGATAACTGGGACAATATTTCTCGCGTTCTGCTTGGCAGAAGAAGGAGAGTCAGAAACATAGGTTTGAAGAAGGAAGACGACGCGGCAGCTGTAGTGAAGATGGTGCTTAACCCTTTCCGTCCCCTTGTGGAGGTGTTTGGCCTGCGTGCTTACCCTAGTTTCAGTGGCACCATCTCCGCCGTGGACGACAAAGGAAGCAAGGTCGTCATTTACAACAAGGACCCTCAAGCCAAACGAGATGATACTCCGTCCTCTCAGACGCAAACTCAGAGTAATATCGATTGTCACCACAAAGAAGCACAGGAGGATTTGCCACCTGCTGTAACGTACAGACCCATCTCAGCTGACGGGGAGTTCTACATTCAAGTCAGTGGCAATTCCACCACACATACCTCCCGTCTCACCTTGGTATGGGATGGTCATTCCGATAAAGCTGCGTACAACATCATCAACACACACCAAATCAGCATAGGCAGCTCCAAAGCTGAACTAACGTATGCGGTGCTCCACGATGCCGTGGAGGCCACCGTGAAGGTCCAGCTGAATCTCAAGGAACCCGAAGAAGCTGGCACCGTCACCGGCGTGTACGGTGAAATCACCGCGACCAACCAGCACTACAAGAACAAGAGCATCCTGCTCTTCAGCCATCCAAAGGAACAGGCAGTAAAACCCGAGGTCAATGCCTCGGATGCCGTTTCAATTTCACTCAAGTTGATGAGGGGTTTCATTGTGGCGTCACTTGAGCGCTTGCTTGTGATAAAGGTTAGCTTGCGTGCTGTGTGCTCCAGCTATGAGGAACCCATATGCTTCGAGCAAGAAGTGAGTTTCGACCCCAATATGGGTACCGCGCAAACAGCGAAGATCGATGCACCCACTATGGAGGCTCAAGTGATGTTCATCACCAACGAGCGAAATGCGCCCAGCCAGGGAAGGGTAAAGCAATGGCGCCGGCGACCACGCGCGAGTGCCGCAATACTCCCCAACACTACTACTGCGGGA

In the KaKs calculator, I use the NG method and get the NA of the ks value. I don’t know what problems they have. What should I do?


KaKs


software error


gene

• 629 views

If the nucleotide and amino acid sequence are correctly aligned, this could be due to a SNP that results in the generation of a stop codon. Hence you are getting a truncated protein.


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