DESeq2 normalization – Found Difference in values when calculated manually
I learnt about how DESeq2 normalises the raw read counts in statquest. It was very helpful and I tried to repeat the steps for my sample data.
But I am getting the output with minor difference – like for each sample outpu I get some difference .
The above is the DESeq2 normalised count which I retrieved using count() in DESeq2 library.
When I manually calculate the normalization I get the following output :
So there is difference . I’m confused why i get this different values ?
Please help me to sort this out..
Many thanks in advance..
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