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Reversible electroporation study of realistic normal and cancerous cervical cells model using avalanche transistor-based nanopulse generator

 September 8, 2021



doi: 10.1088/2057-1976/ac240b.


Online ahead of print.

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Mayank Kumar et al.


Biomed Phys Eng Express.


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Abstract

In this paper, we study the reversible electroporation process on the normal and cancerous cervical cell. The 2D contour of the cervical cells is extracted using image processing techniques from the Pap smear image. The conductivity change in the cancer cell model has been used to differentiate the effects of the high-frequency electric field on normal and cancerous cells. The cells modulate themselves when this high-frequency pulse is applied based on the Debye relaxation relation. To computationally visualize the effects of the electroporation on the cell membrane Smoluchowski equation calculates the number of pores generated and Maxwell equations are used to determine the Transmembrane potential generated on the membrane of the cervical cell. The results produced demonstrates that this mathematical model perfectly describes the numerical tool to study the normal cells and cancerous cells under the electric field. The electric field is provided with the help of a realistic pulse generator which is designed on the principle of Marx circuit and avalanche transistor-based operations to produce a Gaussian pulse. The paper here uses a strength-duration curve to differentiate the electric field and time in nanoseconds required to electroporate normal and cancerous cells.


Keywords:

Electroporation; Reversible Electroporation; Smoluchowski equation; Transmembrane potential; avalanche transistor.

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