What are the rules for the basic statistic of de novo assembly in RNAseq?

Hi all,

I have RNAseq data of non-model organisms, which I’m planning to analyze by de novo assembly with Trinity and write a paper on.

The RNAseq paper contains basic statistics(for example, Total raw reads, Number of gene and Assembly N50 etc).
Are there any rules for these parameters?
I’m a beginner in bioinformatics, so I don’t know what parameters are required.
Can you please advise me?

Thank you very much for your much appreciated help on this!