trinity run_DE_analysis.pl Failed with error: ‘package ‘limma’ could not be loaded’
can someone please help me with this error while running
run_DE_analysis.pl for DESeq2 analysis
Loading required package: edgeR Loading required package: limma Error: package or namespace load failed for ‘limma’: .onLoad failed in loadNamespace() for 'limma', details: call: library.dynam("limma", pkgname, libname) error: shared object ‘limma.dylib’ not found Failed with error: ‘package ‘limma’ could not be loaded’ In addition: Warning messages: 1: package ‘edgeR’ was built under R version 3.6.2 2: package ‘limma’ was built under R version 3.6.2 Error: With R version 3.5 or greater, install Bioconductor packages using BiocManager; see https://bioconductor.org/install Execution halted Error, cmd: Rscript GSE123456.matrix.disease_vs_healthy.DESeq2.Rscript died with ret (256) at run_DE_analysis.pl line 758.
the same is working on other machines but not on this perticular one. DESeq2, edgeR and limma are already installed in R and working . error is same for trinity 13.1 or 13.2.
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