Statistics on RNAseq data
Hi
I would like to know whether you can do statistical tests (e.g. ANOVAS etc.) on the TPM/RPKM counts of RNAseq data?
Thanks
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This is not recommended due to a few underlying problems with RNA-seq data that include compositional bias, library size differences, and correlation between mean and variance. I won’t go into these concepts in detail but use software designed to account for these problems such as DESeq2 or edgeR. Their manuals will have more information on the above concepts.
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