Searching for mRNA ending with a specific 3′ pattern in NON-poly-A RNASeq data.
Hi all,
asking for a colleague,
I’m looking for human non-poly-A mRNA that would end with a specific pattern ( say CCGCAT
).
is it possible to find this in a RNA-SEQ data ? (e.g: www.ncbi.nlm.nih.gov//sra?term=SRR059132 ) ? elsewhere ?
My idea would be to map the RNASeq data, use stringtie and then convert the GTF to fasta.
Is there a better / faster way ?
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