Searching for mRNA ending with a specific 3′ pattern in NON-poly-A RNASeq data.

Searching for mRNA ending with a specific 3′ pattern in NON-poly-A RNASeq data.

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Hi all,

asking for a colleague,

I’m looking for human non-poly-A mRNA that would end with a specific pattern ( say CCGCAT ).

is it possible to find this in a RNA-SEQ data ? (e.g: www.ncbi.nlm.nih.gov//sra?term=SRR059132 ) ? elsewhere ?

My idea would be to map the RNASeq data, use stringtie and then convert the GTF to fasta.

Is there a better / faster way ?


rnaseq


poly-a

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