In Roche 454 next generation DNA sequencing: A) Dideoxynucleosides are used B) Luciferase is used C) DNA is seperated by capillary gel electroparesis D) Reverse transcriptase is used E) All of the above are utilized

Question:

In Roche 454 next generation DNA sequencing:

A) Dideoxynucleosides are used

B) Luciferase is used

C) DNA is seperated by capillary gel electroparesis

D) Reverse transcriptase is used

E) All of the above are utilized

DNA Sequencing:

DNA Sequencing is also acknowledged as chemical sequencing. DNA sequencing works by making use of a technique named electrophoresis. This technique is utilized for separating pieces of DNA on the basis of differences in length as per base.

Answer and Explanation:
1

The correct option is B) Luciferase is used.

Roche 454 is also acknowledged as GS FLX plus. It is described as a pyrosequencing technology-associated sequencing technique. This sequencing displays the use of an enzyme named luciferase.

  • A) Dideoxynucleosides are used: — This is an incorrect answer as dideoxynucleosides are not utilized in this sequencing. It is utilized in the Sangers method.
  • C) DNA is seperated by capillary gel electrophoresis: — This is an incorrect answer as DNA is not separated by capillary gel electrophoresis.
  • D) Reverse transcriptase is used: — This is an incorrect answer as in this type of sequencing, reverse transcriptase is not used.
  • E) All of the above are utilized: — This is an incorrect answer as all the options are not correct.

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