Question:
In Roche 454 next generation DNA sequencing:
A) Dideoxynucleosides are used
B) Luciferase is used
C) DNA is seperated by capillary gel electroparesis
D) Reverse transcriptase is used
E) All of the above are utilized
DNA Sequencing:
DNA Sequencing is also acknowledged as chemical sequencing. DNA sequencing works by making use of a technique named electrophoresis. This technique is utilized for separating pieces of DNA on the basis of differences in length as per base.
Answer and Explanation:
1
The correct option is B) Luciferase is used.
Roche 454 is also acknowledged as GS FLX plus. It is described as a pyrosequencing technology-associated sequencing technique. This sequencing displays the use of an enzyme named luciferase.
- A) Dideoxynucleosides are used: — This is an incorrect answer as dideoxynucleosides are not utilized in this sequencing. It is utilized in the Sangers method.
- C) DNA is seperated by capillary gel electrophoresis: — This is an incorrect answer as DNA is not separated by capillary gel electrophoresis.
- D) Reverse transcriptase is used: — This is an incorrect answer as in this type of sequencing, reverse transcriptase is not used.
- E) All of the above are utilized: — This is an incorrect answer as all the options are not correct.
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