In Roche 454 next generation DNA sequencing:
A) Dideoxynucleosides are used
B) Luciferase is used
C) DNA is seperated by capillary gel electroparesis
D) Reverse transcriptase is used
E) All of the above are utilized
DNA Sequencing is also acknowledged as chemical sequencing. DNA sequencing works by making use of a technique named electrophoresis. This technique is utilized for separating pieces of DNA on the basis of differences in length as per base.
Answer and Explanation:
The correct option is B) Luciferase is used.
Roche 454 is also acknowledged as GS FLX plus. It is described as a pyrosequencing technology-associated sequencing technique. This sequencing displays the use of an enzyme named luciferase.
- A) Dideoxynucleosides are used: — This is an incorrect answer as dideoxynucleosides are not utilized in this sequencing. It is utilized in the Sangers method.
- C) DNA is seperated by capillary gel electrophoresis: — This is an incorrect answer as DNA is not separated by capillary gel electrophoresis.
- D) Reverse transcriptase is used: — This is an incorrect answer as in this type of sequencing, reverse transcriptase is not used.
- E) All of the above are utilized: — This is an incorrect answer as all the options are not correct.
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