‘Can’t connect to eutils.ncbi.nlm.nih.gov:443′
Using an AWS AMI to predict small RNA targets. Getting following error.
=========Prepping RefSeq Files====================
[Wed Sep 8 08:34:12 UTC 2021]
500 Can't connect to eutils.ncbi.nlm.nih.gov:443
No do_post output returned from 'https://eutils.ncbi.nlm.nih.gov/entrez/eutils/efetch.fcgi?db=nucleotide&id=NZ_CP039025&rettype=native&retmode=text&edirect=6.00&tool=edirect&email=ubuntu@ip-172-31-26-35.ec2.internal'
Result of do_post http request is
$VAR1 = bless( {
'_msg' => 'Can't connect to eutils.ncbi.nlm.nih.gov:443',
'_request' => bless( {
'_headers' => bless( {
'user-agent' => 'libwww-perl/6.15',
'content-type' => 'application/x-www-form-urlencoded'
}, 'HTTP::Headers' ),
'_method' => 'POST',
'_uri' => bless( do{(my $o = 'https://eutils.ncbi.nlm.nih.gov/entrez/eutils/efetch.fcgi')}, 'URI::https' ),
'_content' => 'db=nucleotide&id=NZ_CP039025&rettype=native&retmode=text&edirect=6.00&tool=edirect&email=ubuntu@ip-172-31-26-35.ec2.internal'
}, 'HTTP::Request' ),
'_headers' => bless( {
'::std_case' => {
'client-date' => 'Client-Date',
'client-warning' => 'Client-Warning'
},
'client-warning' => 'Internal response',
'client-date' => 'Wed, 08 Sep 2021 08:34:12 GMT',
'content-type' => 'text/plain'
}, 'HTTP::Headers' ),
'_rc' => 500,
'_content' => 'Can't connect to eutils.ncbi.nlm.nih.gov:443
SSL connect attempt failed error:14077410:SSL routines:SSL23_GET_SERVER_HELLO:sslv3 alert handshake failure at /usr/share/perl5/LWP/Protocol/http.pm line 47.
'
}, 'HTTP::Response' );
Cannot retrieve BioProject or BioSample ID for accession NZ_CP039025 at /software/IntaRNA1.0.4/get_refseq_from_ftp.pl line 16.
Cannot open NZ_CP039025.gb
Thanks in advance
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