Deseq2 – how to save zero counts on normalization step
I have raw read counts [of rna-seq]. I need to normalize this data and have done it using DESeq2. But, I found that DESeq2 eliminates the read counts of genes when it’s equal to 0. I don’t want to lose this data as it might affect my result.
Is there any way to do the normalization without eliminating zero read counts?
Thanks in advance.
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