Reads preparation steps for amplicon metagenomics

Reads preparation steps for amplicon metagenomics

0

Hi,

I’m analysing amplicon data for an metagenomics study. I’m wondering if i have to use only read pairs containing primer sequences on the 5′ end allowing some proportion of the mismatches in them of course? I think this step could help to remove PCR artifacts from the real biological data. Is it a useful approach for amplicon metagenomics?


PCR


Metagenomics

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