Ways to analyze treatment changes in scRNAseq
I am working with single cell RNA seq to determine gene expression changes in individual cells using two conditions: untreated and treated. I have merged the untreated and treated files into a single seurat object to generate featureplots of genes that we are interested in, but we are unable to detect strong expression of genes that we have validated in many different assays to be changed after treatment. I wanted to know if there was any way to visualize gene expression changes other than feature plots. for example, could we take the mean expression of our gene of interest in the untreated, and determine fold change in each individual treated cell relative to untreated average? Thanks so much in advance for the help!
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