What is the difference between filtering cells before creating Seurat object and after that?

What is the difference between filtering cells before creating Seurat object and after that?

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I tried filter cells that have unique feature counts over 5000 or less than 350 and > 2% mitochondrial counts before creating Seurat object, and I also tried to do this step after creating Seurat object. The results looked the same. Is there any difference between the two ways that I might not figure out? Which way is the best ?


Seurat


filter


cells

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