Answer: Error during prefetch

Maybe a connection problem, not unusual with NCBI. There is no need to bother with the NCBI Toolkits, you can get fastq files directly with links from e.g.

If you visit that website (which is a wrapper around the NCBI and ENI APIs) and enter your accession it will return several download links, e.g. these to get fastq directly:

so simply do a `wget` or `curl` to get them:


Read more here: Source link