How can I get PHRED score?

How can I get PHRED score?

1

Hi, all.

I am trying to get the assembly stat(Table S1.) according to the following paper about de novo assembly.

[www.ncbi.nlm.nih.gov/pmc/articles/PMC7266049/%5D%5B1]

In the table, there is an item “Mean read PHRED score after filtering and trimming”.
How can I get this?

Is there any way I can calculate the PHRED score based on the fasta file?

I really appreciate your time and effort!


novo


PHRED


Trinity


de


assembly


RNAseq

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updated 1 hour ago by

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written 2 hours ago by

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Is there any way I can calculate the PHRED score based on the fasta file?

You would need fastq files.

You can use FastQC to check the quality of your reads and then create report with MultiQC. It is possible to download a phred scores for all samples from MultiQC in csv or JSON format.

1 hour ago by


MK

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