converting Bam to fastq while removing clipping(hard/soft clip bases)
Hello,
I want to do some analysis and my raw data is paired-end reads fastq files. So far:
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I used BWA mem to convert them to Sam file
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then used samtools to convert to BAM file.
My next step is to convert bam file to fastq file and for that, first I need to exclude the clipping (hard/soft clip bases). Is there any tool that does this while converting to fastq file?
So far, I looked into few tools below:
- samtools: I couldn’t find any option that remove clipping while converting to fastq.
- picard: –CLIPPING_ACTION seems to do this if I set it to ‘X’. But I am not sure.
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