QTL analysis for RNAseq using WASP

Hello
I was advised to do a QTL analysis of our RNAseq data using WASP. I’m been reading about this tool and wondering:

• Would it be possible to do this type of analysis if my RNAseq are all from tumor samples (papilloma and carcinoma of the same tissue) and there’s no control RNA? I think it won’t be possible to do a Combined Haplotype test if we just have 1 type of haplotype (tumor).
• I have 4 carcinoma and 17 papilloma samples. Is that a good size for reliable result with this type of analysis?

Thank you for helping.