Correcting for leukocyt count in QuantSeq analysis
I am conducting a human white blood cell (WBC) QuantSeq analysis. I have heard some stories about the need to correct for WBC count as this could influence the results.
I haven’t been able to find literature about this, so my question is do you really have to correct for WBC count in those kind of analysis? And if so, do you have some literature that I could read?
I can also imagine that this is not necessary because all of your samples are from WBC, so the effects cancel each other out (but you are looking at relative expression anyway).
Any help is appreciated!
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