Exporting normalized counts data from DeSeq2

Exporting normalized counts data from DeSeq2

1

Hi,

I am working with large RNA-Seq dataset downloaded from the NCBI GEO. I would like to export the normalized counts data from DeSeq2 based on size factor normalization for further downstream analysis. It seems like running dds <- DESeq(dds) and dds <- estimateSizeFactors(dds) outputs the same results. Is my understanding correct? Please let me know if I could use any of these to export the normalozed counts data.

library("DESeq2")

dds <- DESeqDataSetFromMatrix(countData = round(Counts_data), colData = sample_info, design = ~ Disease_state)


## 1
dds <- estimateSizeFactors(dds)
sizeFactors(dds)
normalized_counts <- counts(dds, normalized=TRUE)
write.csv(normalized_counts, file="normalized_counts.csv")


## 2
dds <- DESeq(dds)
sizeFactors(dds)
normalized_counts_DESeq <- counts(dds, normalized=TRUE)
write.csv(normalized_counts_DESeq, file="normalized_counts_DESeq.csv")

Created on 2021-09-20 by the [reprex package](reprex.tidyverse.org) (v2.0.1)

Thank you,

Toufiq


rna-seq


DESeq2


normalization


expression

• 51 views

Read more here: Source link