BLAST multiple sequences against each other from one FASTA file
Hi,
I am a beginner to using BLAST locally on Mac OSX. I have downloaded ncbi-blast-2.2.31+.dmg and have installed it. I have a FASTA file with multiple protein sequences in it and am trying to BLAST each sequence against every other sequence within that FASTA file as I am looking for duplicates. I am also a beginner to programming and am struggling to get my head around using the Terminal. I’m not really sure where to start. I’ve tried making a BLAST db from my FASTA file using
makeblastdb -in sequence.fasta -dbtype prot -out sequence -title -sequence
and it seems to have worked but I can’t find the results anywhere.
I’m not even sure this is the right path to take.
Can anyone please help set me on the right path?
Keely
• 4.5k views
Read more here: Source link