Poly A vs total RNA

Poly A vs total RNA – Which is better for extracting noncoding RNA information

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Hi everyone,

In scRNAseq ,I want to extract lncRNA,miRNA etc

Extracted molecule -polyA RNA
or total RNA

Since polyA RNA will not give much information about noncoding counterpart,will it be fruitful to go on with analysis or take a similar study on same tissue and merge them both to get information.

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noncodingRNA


extractedmol


scrnaseq

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