Doing multiple testing correction before or after GO analysis

Forum:Doing multiple testing correction before or after GO analysis

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Hello,

In a given RNA-Seq differential gene expression analysis, one might perform a gene ontology analysis by setting cutoffs to create a gene set (e.g. abs(Log2FC) > 1, p < 0.05). Here, it is possible to use raw p-values (includes more true positive and false-positive DEGs), or FDR-correct p-values (includes less true positives but a smaller-fraction of false positives). Of course, the GO analysis needs to be corrected with FDR as well.

My question is, what are your thoughts for creating gene sets using p-values or q-values? In my mind, any set of filters that are selected A priori should be fine to create a gene set to test for enrichment, and as long as the GO-enrichment tests are corrected using FDR or a similar method, the results are still statistically robust.

Thoughts?


RNA-Seq


testing


comparison


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