Did not take note of my indices (barcodes); is my library salvageable?
Hi everyone. I have a rather annoying problem:
I planned to sequence two small RNA libraries I created a few months ago.
I neglected to write down the indices that I used to create these libraries, and now I am at a loss as to how to continue. Is it possible to perform PCR to detect my barcodes? Can I run these libraries without knowing what the barcodes are right now, and demultiplex later? Or do I need to start over?
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