Makeblastdb error: file does not exist.

Makeblastdb error: file does not exist.


I just downloaded the blast command line applications and I would like to make a database of a MGEs nucleutides that i downloaded from ACLAME database site in fasta file. It is labeled as mydb.fasta and I have put the file into a bin folder of blast in the path C:Program FilesNCBIblast-2.12.0+bin

Ive been typing this into the command line:

cd C:Program FilesNCBIblast-2.12.0+bin to enter in directory

makeblastdb -in mydb.fasta -dbtype nucl -out DB

Building a new DB, current time: 09/23/2021 13:48:15
New DB name: C:Program FilesNCBIblast-2.12.0+binDB
New DB title: mydb.fasta
Sequence type: Nucleotide
Keep MBits: T
Maximum file size: 1000000000B
BLAST options error: File mydb.fasta does not exist

every time i got this error file does not exist. It is probably a simple problem to solve, but I cannot seem to get past it.




Read more here: Source link